乙醇胺修饰的石墨烯量子点的合成及生物成像应用-中国材料进展

文章信息/Info

Title:: One-Pot Synthesis and Bioimaging Application of Ethanolamine-Grafted Graphene Quantum Dots

作者:: 曾敏翔; 陈翔; 谢虞清; 管剑; 甄杰明; 朱先军; 杨上峰; 中国科学技术大学材料科学与工程系，中国科学院能量转换材料重点实验室，合肥微尺度物质科学国家实验室

Author(s):: ZENG Minxiang; CHEN Xiang; XIE Yuqing; GUAN Jian; ZHEN Jieming; ZHU Xianjun; YANG Shangfeng

摘要:: 作为新一代基于碳材料的量子点，乙醇胺（Ethanolamine，ETAM）修饰的石墨烯量子点(ETAM-GQDs)成功地通过一步水热法被合成出来，并通过实验显示出在生物成像应用中的潜力。以柠檬酸作为碳源、甘氨酸作为桥联剂，通过乙醇胺/去离子水共溶剂水热法，成功地实现了在石墨烯量子点表面修饰乙醇胺得到ETAM-GQDs。通过原子力显微镜（AFM），光电子能谱（XPS），拉曼光谱等对ETAM-GQDs进行了表征。在测得ETAM-GQDs的稳态荧光光谱后，通过使用硫酸奎宁作为参比，在365nm紫外光激发下测得的ETAM-GQDs的量子产率为38.2%。除此之外，活体细胞HL7702细胞和ETAM-GQDs共培养后，通过荧光成像实验证实了ETAM-GQDs可以作为有效的生物成像剂。

Abstract:: Ethanolamine-grafted graphene quantum dots (ETAM-GQDs) as a novel type of carbon-based quantum dots have been successfully synthesized via a facile one-pot hydrothermal approach, and show potential application in bioimaging. The hydrothermal synthesis of ETAM-GQDs was accomplished by introducing ethanolamine (ETAM) as the co-solvent blending deionized water while citric acid and glycine are used as the main carbon source and bridge for condensation respectively. The size distribution of ETAM-GQDs was determined by AFM and TEM. The photoluminescent (PL) quantum yield of ETAM-GQDs was measured to be 38.2% under 365 nm light excitation by using quinine sulphate as a standard. The biocompatibility of ETAM-GQDs evaluated by MTT assay indicated the low toxicity of ETAM-GQDs at concentrations below 1 mg/mL. Fluorescent imaging of living HL7702 cells incubated with ETAM-GQDs demonstrated that ETAM-GQDs can be applied as an effective bioimaging agent.