抗菌多肽改性杂萘联苯聚芳醚腈酮研究-中国材料进展

文章信息/Info

Title:: Antimicrobial Surface Functionalization of Poly(arylene ether nitrile)s Containing Phthalazinone Moieties with Polypeptides

作者:: 刘文韬1; 柳承德1; 2; 刘程1; 2; 3; 王锦艳1; 2; 3; 蹇锡高1; 2; 3; （1．大连理工大学化工学院高分子材料系，辽宁大连 116024）（2．精细化工国家重点实验室，辽宁大连 116024）（3．辽宁省高性能树脂工程技术研究中心，辽宁大连 116024）

Author(s):: LIU Wentao1; LIU Chengde1; 2; LIU Cheng1; 2; 3; WANG Jinyan1; 2; 3; JIAN Xigao1; 2; 3; (1.Department of Polymer Science and Materials，Dalian University of Technology，Dalian 116024，China)(2.State Key Laboratory of Fine Chemicals，Dalian University of Technology，Dalian 116024，China)(3.Liaoning Province Engineering Research Centre of High Performance Resins，Dalian University of Technology，Dalian 116024，China)

Keywords:: PPENK; cationic polypeptide; surface modification; cytocompatibility; antimicrobial activity

摘要:: 人工生物材料容易引起各种各样的细菌感染。抗菌多肽因其优异的抗菌性能及不易产生耐药性细菌等优点，被应用于表面改性生物材料。杂萘联苯聚芳醚腈酮（PPENK）因具有优良的力学性能及易于消毒等特点，有望作为聚芳醚类生物材料。采用表面化学改性的方法在PPENK表面固定阳离子抗菌多肽，可赋予其抗菌性能，拓展杂萘联苯聚芳醚材料在生物医用领域的应用范围。采用伯胺引发氨基酸环内酸酐开环聚合的方法，合成结构不同的两种多肽，然后利用点击化学的原理，将多肽固定到PPENK表面。通过核磁共振氢谱表征所合成聚合单体及多肽的结构。利用傅里叶变换衰减全反射红外光谱（ATRFTIR）及X射线光电子能谱（XPS）分别表征材料表面化学结构、元素及含量变化。通过表面接触角测试材料的亲疏水性。采用MTT法对表面接枝抗菌多肽的PPENK进行细胞毒性测试。使用大肠杆菌及金黄色葡萄球菌对表面接枝抗菌多肽的PPENK进行抑菌活性测试。结果表明，点击化学的方法可以将多肽成功固定在PPENK表面，多肽的引入可以提高PPENK材料的亲水性。细胞毒性测试表明，细胞的存活率均超过80%；抑菌活性测试说明，在PPENK表面引入阳离子抗菌多肽后，可以提高材料对细菌的抑制作用，从而达到抗菌的效果。

Abstract:: Antimicrobial functionalization is an effective way to inhibit bacterial growth on biomedical materials. Antimicrobial polypeptides have several features such as high antimicrobial activity and low cytotoxicity. Poly(arylene ether nitrile)s containing phthalazinone moieties (PPENK) is a promising polyarylether biomaterials owing to its mechanical properties and being easy to sterilize. Antimicrobial surface functionalization of PPENK with polypeptides can expand the application of poly(phthalazione aryl ether)s in biomedical field. Two kinds of polypeptides were synthesized by Ncarboxy angydride ring opening polymerization induced by primary amine. Then, the cationic antimicrobial polypeptides were immobilized on PPENK surface by click chemistry method. The monomer and synthesized polypeptides were characterized by NMR spectra. The samples were characterized by ATRFTIR, XPS measurements and contact angle. The cell viability of MC3T3E1 preosteoblast cells on samples were evaluated by MTT assay, and the antimicrobial activity was tested by escherichia coli and staphylococcus aureus. The results showed that the cationic antimicrobial polypeptides were successfully immobilized on PPENK surface. The viability of cells cultured with extracts from polypeptides grafted PPENKs was more than 80%. The introduction of cationic antimicrobial polypeptides on the surface of PPENK could inhibit the growth of bacteria.