(East China University of Science and Technology, Engineering Research Center for Biomedical Materials of Ministry of Education, Shanghai 200237, China)
Keywords:
-
CLC:
PACS:
R318.08
DOI:
10.7502/j.issn.1674-3962.2013.10.04
DocumentCode:
Abstract:
Dextran vinyl sulfone (Dex-VS) and mercapto chitosan (CS-SH) derivates were synthesized and characterized in the study. In addition, CS-SH was selected as a kind of macromolecular crosslinking agent taking advantage of the Michael addition between 朧S and 朣H groups, thus generated dextran-based hydrogel network. Morphology of the hydrogel scaffold was inspected through scanning electron microscope (SEM). Various degradation behaviors under different conditions were investigated. The duration time can be regulated via both concentration of dextran precursors and degree of substitutions. Cellular biocompatibility of the hydrogel was confirmed through MTT assay. Based on the above results, the inductive growth factor, rhBMP-2, was loaded into the scaffold and implanted into mouse thigh muscles to investigate ectopic bone formation. Mass of neo-bone was notable observed compared to control group, as well as the histological evaluations. Consequently, such kind of hydrogel might give promising application in tissue regeneration and delivery of growth factors.