With increasing restrictions of environmental regulations and safety concerns on traditional poisonous coatings, it is highly desirable to develop a new green method for anti-biofouling in marine environment. In this paper, a green method that is high voltage pulse electric field (HPEF) combined with carbon black composite coating was found to inhibit the adhesion of diatom. Results are presented from diatom inactivation assays on the composite coating upon the application of HPEF. Diatom on the composite coating surfaces was visualized using scanning electron microscopy (SEM) for exploring the mechanism of that HPEF inhibit the adhesion of diatom. Using pulses of 10min in duration, 23.15 kHz in frequency, 0.5 in duty cycle and 19 kV in pulse amplitude, diatom inactivation rate reached the maximum. The largest inactivation rate of thalassiosira was 89.2%. The maximum inactivation rate of navicular was 92.1%. What’s more, we find that carbon black could increase the mechanical properties of epoxy resin, and the optimum content of carbon black was 0.3%.