TC16 Alloy wire which has high-precision dimension controlling and meets the special requirement of microstructures and mechanical properties was obtained by cold rolling. In this paper, the effects of cold tandem rolling on M and STA mechanical of TC16 alloy wire were investigated through comparative study of TC16 alloy wire prepared by cold tandem rolling and hot-draw. The results show that the M state tensile and yield strength of TC16 alloy wire obtained by cold tandem were lower, while the ductility was higher, and this benefits the subsequent cold heading formability. The result of solid solution for TC16 Alloy wire prepared by cold tandem rolling was evidenced, the tensile strength has been increased and the ductility has been reduced more drastically. Because the cold tandem rolling can break down elongated phase α of vertical microstructure and store more machining deformation, it makes significant contributions to ductility by annealed and solution strengthening by thermal treatment.