新型镁合金生物材料的体内外相容性研究-中国材料进展

文章信息/Info

Title:: The Compatibility in vitro and in vivo of Novel Biomedical Magnesium Alloy

作者:: 高质涵1; 高铭泽2; 孙艺3; 刘海峰1; 付高洁4; 肖阳4; （1. 牡丹江医学院医药研究中心，黑龙江牡丹江 157011）（2. 牡丹江医学院医学影像学院，黑龙江牡丹江 157011）（3. 东北财经大学工商管理学院，辽宁大连 116000）（4. 牡丹江医学院附属第二医院，黑龙江牡丹江 157011）

Author(s):: GAO Zhihan1; GAO Mingze2; SUN Yi3; LIU Haifeng1; FU Gaojie4; XIAO Yang4; （1. Medical Research Center, Mudanjiang Medical University, Mudanjiang 157011,China）（2. School of Medical Imaging, Mudanjiang Medical University, Mudanjiang 157011, China）（3. School of Business Administration, Dongbei University of Finance and Economics, Dalian 116000, China）（4. The Second Affiliated Hospital, Mudanjiang Medical University, Mudanjiang 157011, China)

Keywords:: magnesium alloy; gallium; strontium; biocompatibility; biodegradation; orthopedic implants

摘要:: 近年来，镁合金材料由于其生物可降解、且机械性能与皮质骨相似而备受关注。探讨了含不同质量分数镓和锶元素的镁合金材料在体内外的相容性及是否具有促进骨愈合的能力。将各组材料在杜氏改良Eagle培养基（DMEM）中与人骨髓间充质干细胞（hBMSCs）共同培养，然后通过荧光染色判断材料在体外的细胞毒性；将材料植入大鼠股骨内并通过X射线透视、MicroCT评价植入材料在体内的相容性以及作用，通过扫描电镜观察植入材料表面形貌的改变。研究结果表明，相较于纯钛，镁合金材料会对细胞增殖产生一定的抑制作用；含锶的镁合金材料具有更好的促成骨能力，镁合金材料的降解速率小于纯镁。

Abstract:: In recent years, magnesium-based alloys have attracted extensive attention due to their biodegradability and mechanical properties similarity with those of cortical bone. This paper investigated the compatibility in vitro and in vivo of magnesium alloys containing different amounts of gallium and strontium and whether they have the ability of promoting bone healing. The materials of each group were co-cultured with human bone marrow mesenchymal stem cells (hBMSCs) in Duchenne modified eagle medium (DMEM), and the cytotoxicity of the materials in vitro was evaluated by fluorescence staining. The materials were implanted into the femur of rats, and X’ray fluoroscopy and Micro-CT were used to evaluate the compatibility in vivo and effect of the planted materials, SEM was used to observe the changes of the surface morphology of the planted materials. Compared with pure titanium, magnesium alloys have a certain inhibitory effect on cells proliferation. The alloy material containing strontium has better bone forming ability, and the degradation rate is lower than that of pure magnesium.