胶原/HA复合涂层多孔钛的细胞响应行为-中国材料进展

文章信息/Info

Title:: Cellular Response on Collagen/HA Composite Coating of Porous Titanium

作者:: 李若琳1; 张云龙1; 寇宏超2; 李仁兴1; 卢雅琳1; (1. 江苏理工学院材料工程学院，江苏常州 213001)(2. 西北工业大学凝固技术国家重点实验室，陕西西安 710072)

Author(s):: LI Ruolin1; ZHANG Yunlong1; KOU Hongchao2; LI Renxing1 ; LU Yalin1; (1. School of Materials Engineering，Jiangsu University of Technology，Changzhou 213001，China) (2. State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xian 710072, China)

Keywords:: porous titanium; hydroxyapatite coating; collagen; cellular response

摘要:: 为提高医用多孔钛的表面活性及生物相容性，以纤维烧结多孔钛为载体，采用碱热处理+模拟体液等化学方法进行羟基磷灰石(HA)沉积，随后进行水溶性胶原涂覆，制备具有三维贯通孔结构的胶原/HA复合涂层多孔钛。研究了胶原/HA复合涂层的表面形貌及化学成分，探讨复合涂层的形成机理；进行体外细胞毒性实验并分析了多孔钛表面细胞响应行为。结果表明，胶原/HA复合涂层在钛纤维表面均匀附着，胶原的填充连接可修复HA涂层表面的微裂纹，有利于小鼠前成骨细胞的黏附、增殖及分化，促进细胞的孔内跨纤维生长。胶原/HA复合涂层可以得到更好的细胞响应，对于促进早期骨与植入体的固定有很大的潜力。

Abstract:: In order to improve the surface activity and biocompatibility of the medical porous titanium, sintered fibrous porous titanium was used as a carrier, the hydroxyapatite (HA) was deposited by alkaline heat treatment and simulated body fluids, then the water soluble collagen was coated on the HA surface to form collagen/HA composite coated porous titanium with threedimensional penetrating pore structure. The surface morphology and chemical composition of collagen/HA composite coating were studied, the formation mechanism of collagen/HA composite coating was discussed. The cytotoxicity in vitro was tested and the cell response behavior on porous titanium surface was analyzed. The results show that the collagen/HA composite coating was uniformly adhered on the surface of the titanium fiber, the microcracks on the surface of HA coating can be repaired by collagen filling, which improves adhesion, proliferation, differentiation of mouse previous osteoblasts and the interfiber growth. HA/collagen composite coating shows an improved cellular response and an promotion of fixation between early bone and implant.