[1]杨高洁,陈俊孚,吴苏州,等.仿生骨组织工程材料的微纳制造与性能研究[J].中国材料进展,2020,(09):691-700.[doi:10.7502/j.issn.1674-3962.201906002]
　YANG Gaojie,CHEN Junfu,WU Suzhou,et al.Micro/Nano-Fabrication and Properties of Biomimetic Bone Tissue Engineering Materials[J].MATERIALS CHINA,2020,(09):691-700.[doi:10.7502/j.issn.1674-3962.201906002]
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仿生骨组织工程材料的微纳制造与性能研究()
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中国材料进展[ISSN:1674-3962/CN:61-1473/TG]

卷:
期数:
2020年第09期
页码:
691-700
栏目:
出版日期:
2020-09-30

文章信息/Info

Title:
Micro/Nano-Fabrication and Properties of Biomimetic Bone Tissue Engineering Materials
文章编号:
1674-3962(2020)09-0691-10
作者:
杨高洁1; 2; 陈俊孚2; 吴苏州2; 李丽坤3; 刘昱4
（1. 华中科技大学材料科学与工程学院，湖北 武汉 430074）（2. 深圳晶莱新材料科技有限公司，广东 深圳 518000）（3. 广东省焊接技术研究所，广东 广州 510650）（4. 华中科技大学生命科学与技术学院，湖北 武汉 430074）
Author(s):
YANG Gaojie1; 2;  CHEN Junfu2;  WU Suzhou2;  LI Likun3;  LIU Yu4
(1. Department of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China) (2. Shenzhen Jinglai New Material Technology Co, Ltd, Shenzhen 518000, China) (3. Guangdong Welding Institute, Guangzhou 510650, China) (4. Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China)
关键词:
仿生; 微纳制造; 成骨分化; 血管化; 骨再生
Keywords:
biomimetic;  micro/nanofabrication;  osteogenesis;  angiogenesis;  bone regeneration
分类号:
R318.08; TN305.7
DOI:
10.7502/j.issn.1674-3962.201906002
文献标志码:
A
摘要:
通过在植入材料表面构建仿生微纳结构，可以为机体细胞提供有利的微环境，从而提高细胞活性并促进组织修复。然而，到目前为止仍没有一种有效的骨组织工程材料能在成分和结构上同时模拟天然骨。因此，采用仿生矿化法复合微纳制造技术在仿生骨修复材料中构建了大规模可控的微纳结构，并且首次在体内证明了该材料的成分和可控有序的微纳结构能够长效促进血管化骨再生。研究结果表明：首先，通过复合高分子模板实现对仿生矿化羟基磷灰石纳米颗粒（HANPs）形貌的调控，而且微接触法将有序的微米图案大规模地整合入骨组织工程材料中；其次，大鼠间充质干细胞（rMSCs）在该仿生骨组织工程材料表面表现出高度取向生长，其成血管分化和成骨分化表达增加；同时，构建的大鼠颅骨缺损模型也证明了该仿生骨组织工程材料能促进血管化膜内成骨。这为控制干细胞定向分化提供了一种先进的研究平台，并为构建基于仿生微纳结构的新型仿生骨组织工程材料提供了有力的理论基础和实际依据，具有潜在的临床应用价值。
Abstract:
Integrating biomimetic micro-nano structure on implants surface can provide a favorable microenvironment for organism cell, so as to effectively increase the cellular activity and improve tissue repairing. However, there are few appropriate bone tissue engineering materials which could simultaneously simulate native bone in the aspect of composition and structure at present. Therefore, biomimetic bone tissue engineering materials with large-scale controllable micro-nano structures were prepared by combining biomineralization and micro/nano-fabrication in this work. Moreover, it was the first time to prove that their composition and controllable ordered micro-nano structure could facilitate the regeneration of vascularized bone. The results have shown that, firstly, mineralized hydroxyapatite nanoparticles (HANPs) with different microstructures were synthesized by different composite polymer templates. And, large-scale ordered micropatterns were integrated on the biomimetic bone tissue engineering materials by micro-contact method. Secondly, rat mesenchymal stem cells (rMSCs) cultured on the surface of biomimetic bone tissue engineering materials present highly orientated alignment. The angiogenesis and osteogenesis expressions of rMSCs increase. Meanwhile, we also found the biomimetic bone tissue engineering materials promoting vascularized intramembranous ossification via a rat skull defect model. This work provides an advanced platform for manipulating directional differentiation of stem cells, as well as gives a theoretical and practical foundation for preparing novel biomimetic bone tissue engineering materials with micronano structures. It is a potential application for this materials in clinic.

备注/Memo

备注/Memo:
收稿日期：2019-06-01 修回日期：2019-11-20 基金项目：国家自然科学基金面上项目（81471792）第一作者：杨高洁，女，1984年生，博士， Email:gaojiey1128@gmail.com
更新日期/Last Update: 2020-08-28