[1]梁瑜,乔勇,刘星志,等.三维成球培养优化间充质干细胞的研究进展[J].中国材料进展,2020,(04):278-286.[doi:10.7502/j.issn.1674-3962.201912014]
 LIANG Yu,QIAO Yong,LIU Xingzhi,et al.Progress in Optimization of Mesenchymal Stem Cells by Three-Dimensional Spheroid Culture[J].MATERIALS CHINA,2020,(04):278-286.[doi:10.7502/j.issn.1674-3962.201912014]
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三维成球培养优化间充质干细胞的研究进展()
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中国材料进展[ISSN:1674-3962/CN:61-1473/TG]

卷:
期数:
2020年第04期
页码:
278-286
栏目:
出版日期:
2020-04-30

文章信息/Info

Title:
Progress in Optimization of Mesenchymal Stem Cells by Three-Dimensional Spheroid Culture
文章编号:
1674-3962(2020)04-0278-09
作者:
梁瑜12乔勇2刘星志23徐中娟23朱小立1索广力2
(1.上海大学生命科学学院,上海 200444)(2.中国科学院苏州纳米技术与纳米仿生研究所,江苏 苏州 215123)(3.中国科学技术大学纳米技术与纳米仿生学院,安徽 合肥 230026)
Author(s):
LIANG Yu12 QIAO Yong2 LIU Xingzhi23 XU Zhongjuan23 ZHU Xiaoli1 SUO Guangli2
(1.School of Life Sciences, Shanghai University, Shanghai 200444, China) (2.Suzhou Institute of NanoTech and NanoBionics, Chinese Academy of Sciences, Suzhou 215123, China) (3. School of NanoTech and NanoBionics, University of Science and Technology of China, Hefei 230026, China)
关键词:
三维培养间充质干细胞干细胞优化临床应用细胞球
Keywords:
three-dimensional culture (3D culture) mesenchymal stem cells (MSCs) stem cell optimization clinical application spheroid
分类号:
R318
DOI:
10.7502/j.issn.1674-3962.201912014
文献标志码:
A
摘要:
间充质干细胞(mesenchymal stem cells, MSCs)源于发育早期的中胚层,因其来源广泛、具有多向分化潜能、低免疫原性和自我更新能力,在组织工程和再生医学应用中显示出巨大的潜力,也是当前基础研究和临床研究中应用最多的一类干细胞。然而,间充质干细胞的临床应用面临许多挑战,比如治疗所需细胞数量巨大,细胞质量存在异质性,细胞体内移植后存活率低,以及二维(two-dimensional, 2D)贴壁培养导致间充质干细胞特征衰减等。三维(three-dimensional, 3D)成球培养可以更好地模拟体内微环境,且大量的研究证明,3D成球培养增强了间充质干细胞的细胞存活和因子分泌能力,促进了干细胞特征维持、细胞迁移和血管生成,在临床医学领域具有广阔的应用前景。基于此,综述了体外3D成球培养的方法、3D成球培养优化的间充质干细胞的生物学特性及应用,并对3D成球培养未来的研究方向进行展望。
Abstract:
As an important branch of stem cell, mesenchymal stem cells (MSCs) are derived from the mesoderm at early embryonic development, which have shown great potentials in tissue engineering and regenerative medicine applications due to their extensive sources, multilineage differentiation potential, low immunogenicity and selfrenewal ability. However, MSCs are still confronting challenges in clinical applications due to requirement of large quantity of cells for therapy, heterogeneity of cell quality, low survival ability after transplantation, the attenuation of MSCs characteristics in twodimensional (2D) culture and ambiguous mechanism of MSCs function for disease therapy. Threedimensional (3D) spheroid culture is widely regarded as a more preferable and closer physiological microenvironment for cells growth. Studies reported that 3D spheroid culture enhanced the characteristics of MSCs on cell survival, factor secretion, stemness maintenance, migration, antisenescence invitro and enhanced the capacities of antiinflammatory, angiogenesis, tissue repair and regeneration invivo. Therefore, 3D culture has broad application prospects in clinical application. In view of this, this article summarized the methods of 3D spheroid culture in vitro, and the spheroid biological characteristics and application briefly. Meanwhile, the prospects in future also have been discussed.

备注/Memo

备注/Memo:
收稿日期:2019-12-22修回日期:2020-03-20 基金项目:国家重点研发计划项目(2017YFA0104301);国家自然科学基金项目(31870975)第一作者:梁瑜,女,1995年生,硕士研究生通讯作者:索广力,男,1971年生,研究员,博士生导师, Email:glsuo2013@sinano.ac.cn
更新日期/Last Update: 2020-03-26