猫须草纳米分散液的制备及其降尿酸活性研究-中国材料进展

文章信息/Info

Title:: Preparation of Orthosiphon Stamineus Nano-Dispersion and Its Effect on Uric Acid

作者:: 刘睿1; 罗超2; 朱君1; 2; （1. 纳米技术及应用国家工程研究中心，上海 200241）（2. 上海健康医学院上海市分子影像学重点实验室，上海 201318）

Author(s):: LIU Rui1; LUO Chao2; ZHU Jun1; 2; （1. National Engineering Research Center for Nanotechnology，Shanghai 200241，China）（2. Shanghai Key Laboratory of Molecular Imaging，Shanghai University of Medicine & Health Sciences， Shanghai 201318，China）

Keywords:: orthosiphon stamineus; nanotechnology; uric acid; liver side effect; cell cytotoxicity

摘要:: 纳米中药是近年来迅速发展起来的前沿科技领域。纳米技术和传统中药制剂的有机结合，有力地推动了医药科技的发展。以猫须草为研究对象，采用剪切、研磨和高压均质等方法制备了猫须草纳米分散液，获得了平均粒径约为100 nm的均匀、稳定的分散体系，研究了纳米分散液降尿酸（UA）的作用。结果表明：相对于高尿酸模型组，纳米分散液对UA的抑制率为22.1%；相对于水提液实验组，纳米分散液表现出更好的降尿酸作用。通过进一步考察其对肝脏指标的影响，探究了纳米分散液的毒副作用。相对于高尿酸模型组，纳米分散液对肝脏谷丙转氨酶（ALT）和谷草转氨酶（AST）的表达水平未见明显影响，这表明纳米分散液未引起明显的肝损伤。最后，通过细胞实验证明了纳米分散液具有较低的细胞毒性。该实验表明猫须草纳米分散液的剂型在降尿酸方面具有潜在的应用价值，为纳米中草药的应用提供了理论依据。

Abstract:: Nano-traditional Chinese medicine is a new research field that is developing rapidly in recent years. The combining of nanotechnology with traditional Chinese medicine produces an enormous influence on the development of medical science. In this paper, the preparation method of orthosiphon stamineus nano-dispersion is developed by shear grinding and high pressure homogenization. A uniform and stable nano-dispersion with average particle size of about 100 nm is obtained. Furthermore, the effect of the nano-dispersion on reducing uric acid (UA) is investigated. Compared with the model group, the inhibition rate of the nano-dispersion on UA is 22.1%, and compared with the water extract experimental group, the nano-dispersion shows a better effect on reducing UA. Additionally, the side effects of the nano-dispersion are evaluated by investigating the liver indexes, which shows that nano-dispersion causes less liver injury through detecting alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of liver compared to that of the model group. Finally, the cytotoxicity of nano-dispersion is proved by cell experiment. The results show that the orthosiphon stamineus nano-dispersion has potential application in reducing UA, which can provide a theoretical basis for the reasearch of nano-dispersion about traditional Chinese medicine.