[1]黄长瑾,雷桓,唐晓军.EDC/NHS交联重组胶原蛋白水凝胶修复关节软骨缺损[J].中国材料进展,2024,43(04):010-19.
　HUANG Changjin,LEI Huan,TANG Xiaojun.Study on the Repair of Articular Cartilage Defects by EDC/NHS Cross-linked Recombinant Collagen Hydrogel[J].MATERIALS CHINA,2024,43(04):010-19.
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EDC/NHS交联重组胶原蛋白水凝胶修复关节软骨缺损()
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中国材料进展[ISSN:1674-3962/CN:61-1473/TG]

卷:
43
期数:
2024年第04期
页码:
010-19
栏目:
出版日期:
2024-04-29

文章信息/Info

Title:
Study on the Repair of Articular Cartilage Defects by EDC/NHS Cross-linked Recombinant Collagen Hydrogel
作者:
黄长瑾; 雷桓; 唐晓军
1. 中国医学科学院北京协和医学院 整形外科医院，北京 100144 2. 西北大学化工学院，陕西 西安 710069 3. 西北大学 生物医药研究院，陕西 西安 710069
Author(s):
HUANG Changjin;  LEI Huan;  TANG Xiaojun
1. Hospital of Plastic and Reconstructive Surgery, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100144, China 2. College of Chemical Engineering, Northwestern University, Xi’an 710069, China 3. Institute of Biomedical Research, Northwestern University, Xi’an 710069, China
关键词:
EDC/NHS; 交联; 重组胶原蛋白; 可注射水凝胶; 关节软骨修复
Keywords:
EDC/NHS;  cross-linking;  recombinant collagen;  injectable hydrogel;  articular cartilage repair
分类号:
R3
文献标志码:
A
摘要:
关节软骨损伤、缺损是一种常见且严峻的临床挑战，没有有效的治疗方法，基于胶原蛋白的水凝胶是软骨组织工程的研究热点之一，但在开发具有良好生物相容性且可安全交联的胶原蛋白产品，并提高软骨修复有效性方面仍然存在巨大挑战。将重组Ⅰ型胶原蛋白和重组Ⅲ型胶原蛋白通过EDC/NHS交联，制备了两种水凝胶。两种水凝胶表现出良好的三维孔隙结构，以及优异的力学性能和可降解性。研究发现，重组Ⅰ型胶原蛋白水凝胶（Gel-I）和重组Ⅲ型胶原蛋白水凝胶（Gel-Ⅲ）能促进细胞糖胺聚糖的分泌，发现Gel-I可以显著上调BMSC细胞表达COLⅡ基因和SOX9基因，Gel-Ⅲ可以显著上调SOX9基因。在兔子膝关节软骨修复模型中，与空白组和Gel-Ⅲ组相比，Gel-I组在骨体积大小方面表现出明显优势，软骨下骨已形成并有效恢复，小梁的数量和厚度最高，小梁分布更均匀，并且缺损处新软骨厚度与相邻软骨厚度相同。总之，重组I型胶原蛋白水凝胶在软骨修复有效性以及安全性方面具有极大的临床应用潜力。
Abstract:
Articular cartilage injuries and defects are a common and severe clinical challenge without effective treatment, and collagen-based hydrogels are a hotspot for cartilage tissue engineering, but there are still great challenges in developing collagen products with good biocompatibility and safe cross-linking and improving the effectiveness of cartilage repair. In this study, recombinant type I collagen and recombinant type III collagen were cross-linked by EDC/NHS to prepare injectable hydrogels. The two hydrogels exhibited a good three-dimensional pore structure, as well as excellent mechanical properties and degradability. It was found that recombinant type I collagen hydrogel (Gel-I) and recombinant type III collagen hydrogel (Gel-III) could promote the secretion of cellular glycosaminoglycans. The effects of the hydrogels on the chondrogenic differentiation of BMSC were analyzed by q-RTPCR, and it was und that Gel-I significantly up-regulated COLII gene and SOX9 gene, and Gel-III significantly up-regulated SOX9 gene. In the rabbit knee articular cartilage repair model, compared with the blank and Gel-III groups, Gel-I group showed the significant advantage in bone volume size, subchondral bone had been formed and effectively restored, the number and thickness of trabeculae were the highest, the trabeculaes were more uniformly distributed, and the thickness of the new cartilage at the defect was the same as that of the neighbouring cartilage. In conclusion, recombinant type I collagen hydrogel has great potential for clinical application in terms of the effectiveness and safety of cartilage repair.

备注/Memo

备注/Memo:
收稿日期：2024-01-06 基金项目：陕西省可降解生物医学材料重点实验室项目（11JS104）；陕西省可降解生物医学材料重点实验室项目（12JS101） 第一作者：黄长瑾，女，1991年生，博士 通讯作者：唐晓军，男，1973年生，教授，博士生导师，Email:frog30@126.com
更新日期/Last Update: 2024-03-28