蛋白酶靶向的荧光纳米诊疗探针研究进展-中国材料进展

文章信息/Info

作者:: 邢洁1; 2; 姚俊列1; 2; 3; 马雪华1; 2; 3; 吴爱国1; 2; （1.中国科学院宁波材料技术与工程研究所宁波慈溪生物医学工程研究所浙江省生物医学材料技术与应用国际科技合作基地中国科学院磁性材料与器件重点实验室医用植介入材料浙江省工程研究中心，浙江宁波 315201）（2.先进能源科学与技术广东省实验室，广东惠州516000）（3.中国科学院大学，北京 100049）

Author(s):: XING Jie1; 2; YAO Junlie1; 2; 3; MA Xuehua1; 2; 3; WU Aiguo1; 2; (1. Zhejiang Biomedical Materials Technology and Application International Science and Technology Cooperation Base, Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for ImplantInterventional Materials, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technologyand Engineering, Chinese Academy of Sciences, Ningbo 315201, China)

Keywords:: nanotechnology; fluorescent probe; matrix metalloproteinases; cathepsin; fluorescence imaging

摘要:: 蛋白酶是生物系统生长发育、营养代谢等活动中必不可少的基础大分子催化物质，是生命医学领域一直以来关注的重点。研究表明，大多数疾病的发生伴随着蛋白酶的表达异常，某些特定蛋白酶成为一些疾病的生理标志物，因此开发蛋白酶响应诊疗探针可对相应的疾病诊断和治疗提供帮助。荧光成像具有实时、无创、空间分辨率高等特性，而纳米诊疗探针通过合理设计可实现蛋白酶响应荧光成像的同时整合其它治疗方式于同一纳米平台，达到成像和治疗一体化的良好效果。总结了靶向基质金属蛋白酶、组织蛋白酶、半胱天冬氨酸蛋白酶、胰蛋白酶和一些其它类型（如成纤维细胞激活蛋白等）蛋白酶的纳米荧光诊疗探针的成像设计原理及生物医学应用，希望促进新型低毒、高性能的蛋白酶响应型纳米荧光探针的开发，并早日实现临床转化。

Abstract:: Protease is an essential basic macromolecular catalyst for the growth and development of biological system and nutritional and metabolic activities. It has always been the focus of life medicine and other fields. Studies have shown that most diseases are accompanied by abnormal expression of proteases, so some specific proteases have become physiological markers of some diseases. The development of protease detection tools can help in the diagnosis and treatment of corresponding diseases. Fluorescence imaging has the characteristics of real-time, noninvasive and high spatial resolution. Through reasonable design, the fluorescent nano diagnosis and treatment probe can realize the response imaging of protease precise targeting. At the same time, based on nano structure, it can combine other treatment methods on the platform to achieve the good effect of imaging and treatment integration. In this review, we summarize the imaging design principles and biomedical applications of nano fluorescent diagnostic probes targeted to matrix metalloproteinases, cathepsins, caspases, trypsin and some other types of proteases such as fibroblast activating protein, hoping to promote the production of new protease responsive nano fluorescent probes with low toxicity and high performance, and realize clinical transformation as soon as possible.