Abstract

Info

Title:: The Preparation of Perfluorocarbon Emulsion and Its Ability of Oxygen Supply to Organism

Author(s):: WEN Zhengfeng1; ZHU Jun2; GUAN Jun1; YAO Yanjie2; LIU Tian2;
HE Dannong2; LIN Zhaofen1; （1. Department of Emergency, Changzheng Hospital, The Second Military Medical University
（2. National Engineering Research Center for Nanotechnology

Abstract:: Artificial pulmonary surfactant (APS)/Perfluorocarbon (PFC) emulsion using APS as a carrier is prepared through highspeed emulsification, the effect of APS/PFC emulsion on cell proliferation and its ability of improving pulmonary oxygenation to seawater drowning rabbits are explored. PFC content of APS/PFOB freezedried powder is 23.54%. A549 cell viability is increased gradually with the increasing concentration of APS/PFOB emulsion under condition of 1% oxygen concentration. About 124% cell proliferation rate is maintained when the concentration is 5 mg/mL. Moreover, it is demonstrated that APS/PFOB emulsion can improve pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery. APS/PFC emulsion has abilities of promoting A549 cell proliferation during hypoxia and improving pulmonary oxygenation to seawater drowning rabbits through pulmonary delivery, it has potential application for improving lung ventilation.