(1.Department of Polymer Science and Materials，Dalian University of Technology，Dalian 116024，China)(2.State Key Laboratory of Fine Chemicals，Dalian University of Technology，Dalian 116024，China)(3.Liaoning Province Engineering Research Centre of High Performance Resins，Dalian University of Technology，Dalian 116024，China)
Antimicrobial functionalization is an effective way to inhibit bacterial growth on biomedical materials. Antimicrobial polypeptides have several features such as high antimicrobial activity and low cytotoxicity. Poly(arylene ether nitrile)s containing phthalazinone moieties (PPENK) is a promising polyarylether biomaterials owing to its mechanical properties and being easy to sterilize. Antimicrobial surface functionalization of PPENK with polypeptides can expand the application of poly(phthalazione aryl ether)s in biomedical field. Two kinds of polypeptides were synthesized by Ncarboxy angydride ring opening polymerization induced by primary amine. Then, the cationic antimicrobial polypeptides were immobilized on PPENK surface by click chemistry method. The monomer and synthesized polypeptides were characterized by NMR spectra. The samples were characterized by ATRFTIR, XPS measurements and contact angle. The cell viability of MC3T3E1 preosteoblast cells on samples were evaluated by MTT assay, and the antimicrobial activity was tested by escherichia coli and staphylococcus aureus. The results showed that the cationic antimicrobial polypeptides were successfully immobilized on PPENK surface. The viability of cells cultured with extracts from polypeptides grafted PPENKs was more than 80%. The introduction of cationic antimicrobial polypeptides on the surface of PPENK could inhibit the growth of bacteria.