[1]彭耿,王靖,刘昌胜.葡聚糖/壳聚糖水凝胶的制备及其作为生长因子载体的研究[J].中国材料进展,2013,(10):599-604.[doi:10.7502/j.issn.1674-3962.2013.10.04]
　PENG Geng,WANG Jing,LIU Changsheng.Preparation of Dextran/Chitosan hydrogel and application on the carriers of growth factor PENG Geng, WANG Jing, LIU Changsheng[J].MATERIALS CHINA,2013,(10):599-604.[doi:10.7502/j.issn.1674-3962.2013.10.04]
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葡聚糖/壳聚糖水凝胶的制备及其作为生长因子载体的研究()
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中国材料进展[ISSN:1674-3962/CN:61-1473/TG]

卷:
期数:
2013年第10期
页码:
599-604
栏目:
特约研究论文
出版日期:
2013-10-31

文章信息/Info

Title:
Preparation of Dextran/Chitosan hydrogel and application on the carriers of growth factor PENG Geng, WANG Jing, LIU Changsheng
作者:
彭耿; 王靖; 刘昌胜
(华东理工大学,教育部生物医用材料工程研究中心,上海 200237)
Author(s):
 PENG Geng;  WANG Jing;  LIU Changsheng
(East China University of Science and Technology, Engineering Research Center for Biomedical Materials of Ministry of Education, Shanghai 200237, China)
关键词:
水凝胶; 组织再生; 葡聚糖; 壳聚糖; 生长因子
分类号:
R318.08
DOI:
10.7502/j.issn.1674-3962.2013.10.04
文献标志码:
A
摘要:
本文合成并表征了砜基葡聚糖（Dex-VS）和巯基化壳聚糖（CS-SH），利用迈克尔加成反应，以巯基化壳聚糖为大分子交联剂，制备了葡聚糖/壳聚糖（Dex /CS）水凝胶，采用扫描电镜（SEM）考察了凝胶支架的形貌，并研究了降解行为。研究发现，水凝胶的降解时间可由前驱物浓度以及砜基取代度来调控。细胞毒性研究表明该水凝胶具有良好的生物相容性。在此基础上，将其负载骨诱导生长因子人重组骨形态发生蛋白-2（rhBMP-2）后植入老鼠肌袋内，评价动物体内异位成骨的情况。通过新生异位骨的成骨量以及组织学评价结果表明，负载rhBMP-2的凝胶支架具有良好的异位成骨效果。本研究表明此类凝胶有望作为一种新型的可注射生长因子载体用于组织修复。
Abstract:
Dextran vinyl sulfone (Dex-VS) and mercapto chitosan (CS-SH) derivates were synthesized and characterized in the study. In addition, CS-SH was selected as a kind of macromolecular crosslinking agent taking advantage of the Michael addition between 朧S and 朣H groups, thus generated dextran-based hydrogel network. Morphology of the hydrogel scaffold was inspected through scanning electron microscope (SEM). Various degradation behaviors under different conditions were investigated. The duration time can be regulated via both concentration of dextran precursors and degree of substitutions. Cellular biocompatibility of the hydrogel was confirmed through MTT assay. Based on the above results, the inductive growth factor, rhBMP-2, was loaded into the scaffold and implanted into mouse thigh muscles to investigate ectopic bone formation. Mass of neo-bone was notable observed compared to control group, as well as the histological evaluations. Consequently, such kind of hydrogel might give promising application in tissue regeneration and delivery of growth factors.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 基金项目：科技部973计划项目（2012CB933600）
第一作者： 彭耿 ，男，1987年生，硕士生通信作者：刘昌胜 ，男，1967年生，教授，博士生导师
更新日期/Last Update: 2013-10-11